To further strengthen the evidence that Notch1 expression is correlated with NKAP in the development of gliomas, immunohistochemical analysis was conducted to examine the immunostaining of both Notch1 and NKAP in the tissue array of 90 patient samples comprising normal brain tissues as well as glioma tissues classified into grade I, II, III, and IV. This evidence concerns the gene NKAP and glioma.