To investigate the time course for induction of these proteins on infection with E7 and whether this was influenced by virus dinucleotide composition, A549 cells were infected at an MOI of 10 with WT, CpG-H R2 and UpA-H R1R2 mutants of E7 and analysed for OAS3, RNAseL and ZAP expression every 2 h post-infection by WB (Figure 13). This evidence concerns the gene RNASEL and infection.