To address if dietary iron content can influence their frequency and phenotype in the colon and their shift from the CD103+ to the CD11b+ subpopulations during colitis, DC subpopulations in colon LPL and IEL fractions were analyzed by flow cytometry in Rag1-/- mice in the steady state and during T cell transfer colitis after exposure to different diets. Here, ITGAM is linked to colitis.