Yet, the differences between TCR data of healthy controls and the youngest melanoma group remained statistically significant (Figure 2B, CD4, Restricted Vβ-gene families, p = 0.0073; CD4, Distinct peaks, p = 0.0010; CD8, Restricted Vβ-gene families, p = 0.0011; CD8, Distinct peaks, p = 0.00075). This evidence concerns the gene CD8A and melanoma.