PYGO2 and breast cancer: In the cell lines, we found miR‐516a‐3p expression was lower in breast cancer cells MCF‐7 and MDA‐MB‐231 than that in the normal breast cell line HBL‐100 (Figure 1C), whereas Pygo2 protein and mRNA expression were higher in breast cancer cells MCF‐7 and MDA‐MB‐231 than that in the normal breast cells HBL‐100 (Figure 1D‐E).