In conclusion, our results demonstrate that the natural molecule kaempferol could effectively attenuate liver fibrosis formation, inhibit HSCs activation and further suppress HSCs collagen synthesis both in vivo and in vitro. In addition, kaempferol could selectively bind to ALK5 and further down‐regulate the TGF‐β1/Smads pathway. Here, TGFB1 is linked to Hepatic fibrosis.