In these studies, it was observed that HSPB8 protein is induced in response to estrogen stimulation of MCF7 and T47D (estrogen-dependent cell lines) breast cancer cells, whereas it was neither detected nor induced in the triple negative highly invasive MDA-MB231 breast cancer cell lines; overexpression caused a modest accumulation of cells in G2/M54. The gene discussed is HSPB8; the disease is breast cancer.