Even when comparing primary to metastasis within the Moffitt TCC data set as well as in the GSE6919 dataset (196 primary and 75 metastatic samples), HSPB8 mRNA levels were significantly lower, (log2 fold change −1.952; p value = 6.76E-15) (Fig. 7f; Supplementary Fig. 2d, Supporting Data Table 10). This evidence concerns the gene HSPB8 and tarsal-carpal coalition syndrome.