For the Asian lineage ZIKV (P6-740), we detected 5.4 log10 FFU/mL or 4.8 log10 FFU/mL in HuH-7(WT) and HuH-7(HA-EMC4) cell lines, respectively; however, no increase in infectious ZIKV was observed over the time course in EMC4 KO cells (Fig. 3B), indicating that EMC4 was absolutely required for productive ZIKV infection. This evidence concerns the gene EMC4 and Zika virus infectious disease.