To validate the function of Snail2 in LC cells, we further established stable Snail2-overexpressing cell lines using poorly metastatic A549 cells (designated as A549-Snail2) and also knocked down the expression level of Snail2 in the highly metastatic H1299 cells (designated as H1299-sh-Snail2). Here, SNAI2 is linked to laryngotracheoesophageal cleft.