In order to verify whether the observed co-expression trend in EphB4 and autocrine IGF-II in cancer cell lines was functionally correlated, we set up an experiment using three IGF-II secreting malignant mesothelioma cell lines (NCI-H28, NCI-H2052, and MSTO-211H). The gene discussed is EPHB4; the disease is malignant mesothelioma.