Then, RT-qPCR and Western blot analysis were performed to confirm the interaction between miR-30b-3p and RECK, finding that mRNA and protein levels of RECK were decreased in glioma cells after overexpression of miR-30b-3p but increased in glioma cells after depletion of miR-30b-3p (Figure 3B,C). Here, RECK is linked to central nervous system cancer.