Because cancer cells have the properties of uncontrolled cell proliferation and dysfunction of cell cycle checkpoint, we analyzed the cell cycle-related genes/proliferation marker genes G1/S-specific cyclin-E1 (ccne1), cyclin-dependent kinase 1 (cdk1), and cyclin-dependent kinase 2 (cdk2) by qPCR. The gene discussed is CCNE1; the disease is cancer.