CCNB1 and osteosarcoma: The data showed that the protein level of phospho-cdc2, cdc2, and Cdc25C were decreased in treated both cell lines, but Cyclin B1 protein levels had no apparent change in HOS cells and was decreased in MG-63 cells (Figure 3C), indicating Licochalcone A induces G2/M phase arrest of osteosarcoma cell lines.