IHC staining of H1299control primary tumours supported these results as NANOG and high levels of nuclear YAP1 are apparent in H1299control tumours, while the active Hippo pathway in H1299RASSF1A tumours retains the majority of YAP1 in the cytoplasm and no NANOG staining is discernable (Fig 5E). This evidence concerns the gene NANOG and neoplasm.