To address the physiological role of RASSF1A and its involvement during cancer progression, we constructed isogenic H1299 human lung adenocarcinoma cell lines (where RASSF1A is highly methylated) and transfected either with pcDNA3, referred as H1299control, or stably expressing RASSF1A, referred as H1299RASSF1A (Fig 1B). Here, RASSF1 is linked to cancer.