The results showed that among these clinical correlations, ANO1 positive status was significantly correlated with tumor size, the positive rate of ANO1 expression was 81% in the group with tumor size >10 cm, the positive rate of ANO1 expression was 78% in the group with tumor size 5–10 cm, and the positive rate of ANO1 expression was 48% in the group with tumor size ≤5 cm. This evidence concerns the gene ANO1 and neoplasm.