As shown in Fig. 1A, the ANO1 mRNA expression (with an average value of 3 × 10−3) in the GIST group relative to the expression of the housekeeping gene ß-actin was approximately 4-fold higher than the ANO1 mRNA expression (with an average value of 8.5 × 10−4) in the control group. The gene discussed is ANO1; the disease is gastrointestinal stromal tumor.