To further validate the tumor promotion roles of OTUD3 in vivo, we evaluated the effect of OTUD3 on tumor growth in nude mice and found OTUD3 knockdown shrinked tumor growth (Fig. 4f, h and Supplementary Fig. 4e, f) whereas OTUD3 overexpression accelerated tumor growth in vivo (Fig. 4g, i and Supplementary Fig. 4g, h). This evidence concerns the gene OTUD3 and neoplasm.