In consideration of the opposite expression of OTUD3 in different types of cancer, we next used human breast cancer cell MCF7, MDA-MB-231, hepatocellular cancer cell HepG2, colon cancer cell HCT116, and cervical cancer cell HeLa to further investigate the functions of OTUD3 in tumorigenesis and tumor metastasis. This evidence concerns the gene OTUD3 and breast cancer.