In consideration of the opposite expression of OTUD3 in different types of cancer, we next used human breast cancer cell MCF7, MDA-MB-231, hepatocellular cancer cell HepG2, colon cancer cell HCT116, and cervical cancer cell HeLa to further investigate the functions of OTUD3 in tumorigenesis and tumor metastasis. The gene discussed is OTUD3; the disease is cancer.