For this reason, investigators have explored therapeutic benefits of restoring nNOS in mouse models of DMD by overexpressing nNOS via transgenic approach (Wehling et al. 2001; Wehling-Henricks et al. 2005; Wehling-Henricks and Tidball 2011; Rebolledo et al. 2016), and adeno-associated virus (AAV)-mediated gene therapy (Lai et al. 2014). This evidence concerns the gene NOS1 and Duchenne muscular dystrophy.