There were no differences between the levels of ITSN1 proteins in frontal and temporal cortices of AD patients compared with the controls (unpaired two-tailed t-test; n=6–10 for control frontal cortex and n=6–12 for AD frontal cortex; n =8–10 control temporal cortex, n=11–22 for AD temporal cortex (Figure 1a–1d). This evidence concerns the gene ITSN1 and Alzheimer disease.