Neutrophil elastase concentration is high in BD patients and causes tissue injury; therefore, the aim of the present study is to evaluate the damage of epithelial cells after 4- and 24-hour incubation by measuring cytokines IL-1β and IL-8 and TNF-α released when stimulating reconstructed oral mucosal model cell lines with different concentrations of neutrophil elastase (NE). This evidence concerns the gene TNF and Behcet disease.