After analyzing the overall staining intensity, we found that GnRH immunostaining was very weak in normal and early-stage pancreatic cancer specimens (I and II), whereas the high-expression of GnRH was observed in the advanced pancreatic cancer specimens (II, III, and IV), suggesting that GnRH expression might be related to the malignancy in pancreatic cancer (Figure 1B). Here, GNRH1 is linked to pancreatic neoplasm.