Hence, a new model was built by constitutively expressing Notch pathway molecules (RBPJ, MAML1, γ-Secretase complex), JAK2/STAT3 and YY1 proteins and by mutating P53 protein in the GSCs to simulate the differentiation of primary GBM (Grade-IV) cells, and observed how much the model simulation having these higher number of mutations induce the development of GBM cells. Here, RBPJ is linked to glioblastoma.