Accordingly, 2 decades later, Ishii et al. established a uremic model in Sprague-Dawley rats by a single peritoneal injection of 5 mg/kg adriamycin and found that GFR and serum 25-(OH) D were reduced by 52% and 77%, respectively, whereas PTH experienced a 2.5-fold increase after 14 weeks; moreover, the adriamycin-injected rats could also develop low-turnover ROD resembling osteomalacia, as evidenced by a 4.3-fold increase in osteoid volume, a 73% reduction in BFR, a 56% decrease in adjusted apposition rate, and a five-fold increase in mineralization lag time34. This evidence concerns the gene PTH and osteomalacia.