These CRC tissues from the patients whose pathological characteristics showed that TNM (I + II) staging accounted for 58.9, 63.3% were in N0 of lymph node metastasis and M0 of distant metastasis, 86.7% patients were belonging to pathological class I + II, and there was a significant difference of BTF3 expression in different TNM staging between CRC and ANCT (Fig. 2a and Table 1). The gene discussed is BTF3; the disease is metastatic malignant neoplasm in the lymph nodes.