To investigate more detailed the effects of BTF3 expression on the development of colon cancer, BTF3 expression was successfully silenced by LV-BTF3-siRNA transfected into HCT116 cells based on pre-screen results of siRNA sequence efficacy (Fig. 3a) and confirmed that LV-BTF3-siRNA successfully inhibited endogenous BTF3 expression in the cells (Fig. 3b). This evidence concerns the gene BTF3 and malignant colon neoplasm.