To address the potential role of BIN1 in AD-associated Tau pathology, we purified EVs from 20 samples of CSF from individuals with different Braak’s Stages and analyzed an initial 10 samples by WB demonstrating the presence of several BIN1 isoforms in all samples, as calibrated using lysates of HEK293T cells transfected with isoforms 1, 6, 9 and BIN1-13 (BIN1 isoform lacking exons 7, 13, 14, 15, 16 and 17, according to the new nomenclature) expressing plasmids (Fig. 1B). This evidence concerns the gene BIN1 and Alzheimer disease.