We compared the expression of the corresponding transcripts (RNA-Seq) in tumor biopsies from ERBB2+ breast cancer patients22,23 with matched EZH2 protein expression data, revealing a strong negative correlation between EZH2 protein expression and transcript levels of PRC2 target genes from the ErbB2+ GEMM (Supplementary Fig. 2g), consistent with PRC2-mediated repression of these genes. This evidence concerns the gene EZH2 and breast cancer.