However, the behavioral measures of general activity and anxiety in 6–8 week old mice were not predictive of susceptibility to HFD-induced obesity whereas the inflammatory markers IL-6, MPO, CXCL1, and LCN-2 showed only very limited ability in discriminating proneness to DIO when measured before, during, or after administration of HFD. Here, MPO is linked to obesity due to melanocortin 4 receptor deficiency.