Firstly, we identified that for efficient CTL priming, and lysis of relevant cell targets, the presence of CVA21 was required (Fig. 4Ai and Bi); moreover, primed-CTL were tumor specific, as only relevant, but not irrelevant, target cells were capable of stimulating intracellular IFN-γ production (Fig. 4Aii and Bii). The gene discussed is IFNG; the disease is neoplasm.