Although blocking PI3K or mTOR alone by LY294002 and rapamycin, respectively, may efficiently suppress the expression of cyclin Ds and the phosphorylation of Akt, p70S6k and 4EBP1 in certain NSCLC cells, the suppressive effect by co-blockage of PI3K and mTOR was more comprehensive in NSCLC. This evidence concerns the gene PIK3CA and non-small cell lung carcinoma.