Multivariate analysis confirmed the prognostic value of TWIST1 overexpression in combination with N2-3, and in combination with HR(−) status in the EpCAM(+) CTC fraction for the prediction of OS, independently from patients’ age, tumor T stage, grade, nodal status, and the status of the receptors ER, PR, HER2 of the primary site (Table 3). Here, ESR1 is linked to neoplasm.