Multivariate confirmed the prognostic value of HR(−)/TWIST1(+) and TWIST1(+)/N2-3, in the EpCAM(+) CTC fraction for the prediction of DFI (Table 3) independently from patients’ age, tumor T stage, grade, nodal status alone and the HR, and HER2 status of the primary tumor. This evidence concerns the gene ERBB2 and neoplasm.