Similar to our results, recently, Emprou C et al. have shown that in frozen NSCLC tumor samples TWIST1 is more frequently overexpressed in the N+ group compared to the N0 group showing that partial EMT is involved in lymph node progression in early stages of NSCLC [43], while in primary breast cancer loss of E-cadherin is correlated with more than 3 LN involved in 80% of the patients [44]. Here, TWIST1 is linked to neoplasm.