In all EpCAM(+) fractions isolated from 100 early BrCa patient samples and 19 HD CD24, CD44, ALDH1, HPRT transcripts were quantified by the developed quadraplex RT-qPCR and TWIST1 transcripts by the singleplex RT-qPCR assay (Figure 1). The gene discussed is CD24; the disease is invasive breast carcinoma.