In all EpCAM(+) fractions isolated from 100 early BrCa patient samples and 19 HD CD24, CD44, ALDH1, HPRT transcripts were quantified by the developed quadraplex RT-qPCR and TWIST1 transcripts by the singleplex RT-qPCR assay (Figure 1). This evidence concerns the gene HPRT1 and invasive breast carcinoma.