TMEM175 and infection: We generated adeno-associated virus 8 (AAV8) expressing empty vector (EV) and WT TMEM175. Following preinfection with increasing multiplicity of infection (MOI) of AAVs, rat primary hippocampal neurons were incubated with PFF for 14 days, after which they were evaluated for the intensity of p-α-syn inclusions by immunocytochemistry.