To investigate the role of elevated ETHE1 levels as a driver of mitochondrial respiration, we used the Seahorse XF96 extracellular flux analyzer on CRC cells with elevated, normal and knocked-down levels of ETHE1. Using this approach, we generated mitochondrial bioenergetics profiles from HCT116 (Figure 3A) and HT29 (Figure 3B) cells. The gene discussed is ETHE1; the disease is colorectal carcinoma.