In addition, it seems necessary to extend the present study to a larger cohort of human subjects, possibly by using innovative methods to achieve cell-sorting, and to add different preclinical experimental models of obesity to further characterize the epigenetic regulation of CNR1 and OPRM1 expression, as well as the relationship between their peripheral and central modulation. The gene discussed is OPRM1; the disease is obesity due to melanocortin 4 receptor deficiency.