Gene set variation analysis (GSVA) performed on differentially expressed genes for each cluster showed the enrichment of IFNγ signaling and IL-12 signaling mediated by STAT4 in clusters enriched in Csf3r+/+ sarcomas (clusters 2 and 4) (Figure S6B), in line with data obtained by flow cytometry (Figure 3). This evidence concerns the gene IFNG and sarcoma.