Circulating tumor cells enumeration using the CellSearch® and PIK3CA mutational analysis in plasma and DNAs isolated from CellSearch® cartridges were performed in a subgroup of these patients, using the same blood draw (same date and time), more specifically: (a) in 56 samples from 43 patients with early BrCa, (b) in 27 samples from 16 patients with metastatic BrCa, and (c) in 3 PB samples from healthy blood donors. This evidence concerns the gene PIK3CA and invasive breast carcinoma.