When PIK3CA hotspot mutations were analyzed in CTCs isolated using a label‐free technology and corresponding ctDNA in 9 patients using 23 identical blood draws with colorectal cancer, mutational status of PIK3CA showed a match between CTCs and ctDNA in six blood draws and discordance in two, with 15 blood draws showing no mutations; however, in this study, PCR‐based Sanger sequencing (analytical sensitivity 7.5%) was used for mutation detection in CTCs and targeted panel NGS for cfDNA (Kidess‐Sigal et al., 2016). Here, PIK3CA is linked to colorectal cancer.