The colon cancer cells responding to TGFα ligand were determined by DNA sequencing to harbor oncogenic mutations (KRASG13D and BRAFwt in CC36; KRASwt and BRAFV600E in CC14, KRASG12D, and BRAFwt in LS174T) downstream of the TGFα receptor EGFR. This evidence concerns the gene TGFA and malignant colon neoplasm.