Induction of senescence following SETD1A-KD is observed in all cell lines tested (breast cancer, N = 4 cell lines; lung, N = 2 cell lines; colon, N = 7 cell lines), independent of the mutational status of TP53, RB, K-Ras and INK4A, known mediators of cellular senescence signals1,2,25 (Fig. 2c, Supplementary Fig. 2b; Data from three independent experiments are presented as Mean + SD; *p < 0.05 by two-tailed unpaired Student’s t test). This evidence concerns the gene RB1 and breast cancer.