Finally, a triple immunofluorescence was carried out in amygdala with Aβ1–42 (Fig. 5a, b, red) and two of the proteins observed exclusively in AD samples (RNF213; Fig. 5c, d, green) and non-AD samples (CNTN1; Fig. 5e, f, purple). This evidence concerns the gene RNF213 and Alzheimer disease.