In the present study, we determined the autophagy induction capacity of JQ1 in BC and explored the effects of JQ1 on BC cell proliferation both in vitro and in vivo, moreover, we further identified whether autophagy induced by JQ1 modulates BC cell proliferation and the mechanism of how JQ1 induced autophagy, mainly focusing on LKB1/AMPK signal pathway. Here, STK11 is linked to breast cancer.