TP53 and ovarian carcinoma: After evaluating BRCA1/2 and p53 mutation status and CD44 expression levels in HGSOC and other ovarian cancer cell lines by western blot (Figure 2a,b), we elected to further study OVCAR8 and COV362, which carry due to promoter hyper methylation and BRCA1 mutations47 and have high and moderate CD44 expression levels, respectively.