We found that maternal HF diet was associated with increased mRNA expression of genes involved in EGC maturation (Bfabp, Plp1, and S100b), gut barrier function (Lyz1 and Lyz2), and gut barrier integrity (Cldn-3 and Cldn-7) in the male fetal gut (p < 0.05, Figure 9, Figure 10 and Figure 11) compared to CON. Here, PLP1 is linked to hydrops fetalis.