A univariate analysis was performed in this validation cohort, as in the multicenter cohort: Rai stage, LDH, ZAP70 and β-2-microglobulin resulted significant for PFS and TFS; age, LDH and β-2-microglobulin for OS; LDH and β-2-microglobulin for CLL-specific survival. The gene discussed is ZAP70; the disease is B-cell chronic lymphocytic leukemia.