Moreover, analysis of the expression of several immunosuppressive membrane-bound molecules (such as CD80, CD86, PDL1, PD1, Fas-L, CD40L and OX40L) and secretion of various cytokines (such as IL-10, TGF-β and IL-35) by Bregs in specific tumor settings and stages of cancer has revealed functional complexity among the subsets of tumor-evoked Bregs [20]. This evidence concerns the gene CD40LG and neoplasm.