Next, we applied medium B which consisted of Advanced DMEM/F12 (1:1 medium) supplemented with 100 ng/mL EGF, 10 μM Y27632, 5 μM SB202190, 10 mM nicotinamide, 500 nM A-83-01, 2% (v/v) B27, 1% (v/v) Glutamine and 5% (v/v) conditioned medium containing Wnt3A, RSPO1 and Noggin to the next nine ovarian tumor specimens from HOV31T to HOV39T including one germ cell tumor. Here, EGF is linked to testicular germ cell tumor.