In addition, the enhanced migration and invasion induced by gemcitabine and VPA co-treatment were suppressed after STAT3 inhibition, which further verified that STAT3 signaling participates in mediating Bmi1 expression and the biological effect induced by VPA and gemcitabine treatment on pancreatic cancer cells induced by induced by VPA and gemcitabine treatment on pancreatic cancer cells. This evidence concerns the gene BMI1 and familial pancreatic carcinoma.