In a study involving healthy donors, a higher frequency of DENV-specific CD4+ TEMRA that produce more IFNγ than CD4+ TEM or TCM cells and express CXCR3 and cytolytic molecules (e.g., granzyme B and perforin) was found in the blood of individuals classified as primary or secondary infections compared with dengue-negative healthy individuals (53). This evidence concerns the gene CD4 and infection.