To determine if the increased lymphatics (PDPN) that we observe in our patients with PPBC exhibit upregulation of PD-L1 and/or whether PD-1+ T cell infiltration occurs in the tumor microenvironment (TME) of PPBCs, we utilized multispectral imaging of the peri-tumor region in tissues from three different patients with PPBC, who were within 1-year (PPBC1), 3 years (PPBC2), and 4 years post-partum (PPBC3) (Supplemental Table 1). This evidence concerns the gene CD274 and neoplasm.