ANK2 and Alzheimer disease: Because hypermethylation of human ANK1 has been reported in AD cortex (Lunnon et al., 2014), we used RNAi to knock-down expression of Ank1 and Ank2 comparing the effect of reducing these genes in all Drosophila neurons using two different RNAi transgenes designed to non-overlapping regions of each gene.