A selective PFKFB4 inhibitor, 5-(n-(8-methoxy-4-quinolyl)amino)pentyl nitrate, suppressed the glycolysis process and proliferation in human cancer cell lines rather non-transformed epithelial cells in vitro, suggesting that targeting PFKFB4 may be a promising therapeutic strategy against breast cancer. Here, PFKFB4 is linked to breast carcinoma.