Healthy control peripheral blood mononuclear cells were stimulated with IFNα, IFNω or IFNγ in the presence of APS1 patient or healthy control serum. We used the same serum concentration (10%) as previously applied to quantify interferon neutralizing autoantibodies in Gupta et al. (2016). The gene discussed is IFNG; the disease is autoimmune polyendocrine syndrome type 1.