The main objective of the present study was to assess the association of the overexpression of p16 IHC and progression of cervical intraepithelial neoplasia (CIN) 1 to CIN2 or worse (CIN2+) or recurrence of CIN2+ after treatment during follow up in women who had a colposcopy-directed biopsy (CDB). The gene discussed is CDKN2A; the disease is cervical intraepithelial neoplasia.